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Week - 1 |
History and development of plant tissue culture, its applications, advantages and disadvantages |
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Week - 2 |
Basic techniques: Sterilization, Nutrition mediums, Stock solutions, Plant growth hormones, Culture media |
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Week - 3 |
Seed and embryo cultures and applications |
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Week - 4 |
Calluc cultures and applications |
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Week - 5 |
Organ cultures and applications |
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Week - 6 |
Cell and protoplast culture and applications |
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Week - 7 |
Micropropagation and somaclonal variation |
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Week - 8 |
Secondary metabolite synthesis and regulation through plant tissue culture techniques |
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Week - 9 |
Biotransformation, bioreactors,Introduction to plant molecular biology, molecular structure of DNA, genes and chromosomes, protein synthesis |
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Week - 10 |
Isolation of genetic material from plants, gel electrophoresis, sequence |
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Week - 11 |
PCR (Polymerase chain reaction), RFLP, ELISA (Enzyme linked immunosorbant Assay |
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Week - 12 |
Cloning, restriction enzymes, vectors, cDNA |
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Week - 13 |
Blotting, gene transfer and applications |
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Week - 14 |
Applications of molecular techniques for secondary metabolite production |
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