Week - 1 |
Cultivation of Culture Cells to be Used in Toxicity Tests |
Week - 2 |
Obtaining and cultivation of primary culture cells |
Week - 3 |
Cultivation of cell lines |
Week - 4 |
Obtaining and cultivation of stem cells from different sources |
Week - 5 |
Cytotoxicity and Cell Proliferation Methods: MTT, MTS, XTT, WST assays |
Week - 6 |
ATP, Trypan blue, LDH assays |
Week - 7 |
Neutral red, Sulforodamine B, Alamar blue assays |
Week - 8 |
BrdU, Glucose-6 phosphate dehydrogenase, Resasurine, Picogreen assays |
Week - 9 |
Clonogenic assays; Genotoxicity Methods: Chromosome aberration assay |
Week - 10 |
Micronucleus assay |
Week - 11 |
COMET assay |
Week - 12 |
Sister chromatid exchange assay, γ-H2AX focus assay |
Week - 13 |
In Vitro Epithelial Barrier Models in Toxicity |
Week - 14 |
Use of Stem Cells in Toxicological Studies |