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Week - 1
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Cultivation of Culture Cells to be Used in Toxicity Tests |
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Week - 2
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Obtaining and cultivation of primary culture cells |
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Week - 3
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Cultivation of cell lines |
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Week - 4
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Obtaining and cultivation of stem cells from different sources |
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Week - 5
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Cytotoxicity and Cell Proliferation Methods: MTT, MTS, XTT, WST assays |
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Week - 6
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ATP, Trypan blue, LDH assays |
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Week - 7
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Neutral red, Sulforodamine B, Alamar blue assays |
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Week - 8
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BrdU, Glucose-6 phosphate dehydrogenase, Resasurine, Picogreen assays |
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Week - 9
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Clonogenic assays; Genotoxicity Methods: Chromosome aberration assay |
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Week - 10
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Micronucleus assay |
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Week - 11
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COMET assay |
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Week - 12
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Sister chromatid exchange assay, γ-H2AX focus assay |
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Week - 13
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In Vitro Epithelial Barrier Models in Toxicity |
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Week - 14
|
Use of Stem Cells in Toxicological Studies |
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